Hif1 troublesome western blot
WebHIF-1α induces glycolytic reprograming in tissue-resident alveolar macrophages to promote cell survival during acute lung injury. View PDF. In eLife on 13 July 2024 by Woods, P. S., Kimmig, L. M., et al.. Web12 de mai. de 2024 · Western blot assay indicated that the expression of the identified proteins was inhibited in cells expressing D/N-ATF5 and shHIF1α in hypoxia (Fig. 6b). In addition, dual-luciferase assay showed that cells expressing D/N-ATF5 and shHIF1α had similarly lower ability to activate the HIF1 target gene promoters compared with negative …
Hif1 troublesome western blot
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WebWestern blot analysis of HIF1A in A) HepG2 whole cell lysate, (untreated), B) HepG2 whole cell lysate, (200 µM CoCl2 treatment for 24 hr), C) HepG2 whole cell lysate, (500 µM CoCl2 treatment for 24 hr) using HIF1A polyclonal antibody (Product # PA5-85494) using 30 µg of sample at a dilution of 1:5000. WebWestern blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha/HIF1A knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human HIF-1 alpha/HIF1A Monoclonal Antibody (Catalog # MAB19351) followed by HRP-conjugated …
WebIntroduction. The first step in a western blotting procedure is to separate the macromolecules in a sample using gel electrophoresis. Subsequently, the separated molecules are transferred or blotted onto a second matrix, generally a nitrocellulose or polyvinylidene difluoride (PVDF) membrane. WebHey Friends,Western Blot is a technique to determine whether a specific protein is present in a sample. Before, the proteins were separated according to thei...
Web1 de set. de 2011 · Hypoxia-inducible factor-1α (HIF-1α) is a widely studied protein with significant biomedical impact. Care is needed to stabilize HIF-1α protein during sample … Web27 de mar. de 2012 · RT-PCR and Western blot analysis indicated that hypoxia could up-regulate the expression of HIF-1α on both mRNA and protein levels. Hypoxic microenvironment stimulated by CoCl2 could effectively induce apoptosis and influence cell proliferation in PC-2 cells, the mechanism could be related to up-expression of HIF-1α.
WebMA1-515 detects aryl hydrocarbon (Ah) receptor nuclear translocator (ARNT) from human, mouse, rat, and primate tissues. MA1-515 has been successfully used in Western blot, immunofluorescence, gel shift, immunocytochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects an 87 kDa protein representing ARNT in COS cell ...
WebIn Western blotting, I detected clear lower molecular band in about 40-80kDa. On the other hand, Higher molecular band was also detected. I studied that theoretical MW of HIF-1 … how do you calculate a loan origination feeWebImmunoprecipitation of HIF1 alpha protein from HepG2 whole cell extracts treated with 500 μM CoCl2 for 24 hr using 5 μg of HIF1 alpha antibody (GTX127309). Western blot analysis was performed using HIF1 alpha … how do you calculate a rebatehow do you calculate a prorated salaryWebCollect cells into PBS by centrifugation (non-adherent cells) or scraping from culture flasks followed by centrifugation (adherent cells). Wash cells 2X with ice cold PBS at 1000 x g for 10 minutes. Gently resuspend washed pellet in 500 μl Hypotonic Buffer (recipe below). Incubate on ice for 15 minutes. how do you calculate a rolling averageWeb15 de fev. de 2001 · Western blot analysis (Figure 2B) clearly showed that HIF-1α started to accumulate after 1 hour, reached the maximum at 2 hours, and decreased at 4 hours when activated by 100 μM GSNO. In contrast, when 1 mM GSNO was employed, HIF-1α was absent after 1 hour, increased slightly after 2 hours—albeit to a lesser extent … how do you calculate a monthly averageWebIn Western blotting, I detected clear lower molecular band in about 40-80kDa. On the other hand, Higher molecular band was also detected. I studied that theoretical MW of HIF-1 … how do you calculate a scaled scoreWebWestern blot analysis of HIF-1α on lysate from HeLa stimulated with CoCl2, cobalt chloride. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the Mouse Anti- Human HIF-1α … how do you calculate a third