WebDec 14, 2024 · Trisodium phosphate, commonly called TSP, is a packaged, blended cleaning product consisting of 75 to 80 percent trisodium phosphate and 20 to 25 percent sodium … WebApr 26, 2024 · A previous report on alanine-substituted variants of Tsp of E. coli demonstrated Tsp variants with S430A, and K455A substitution lost their catalytic activity . Another report demonstrated the deletion of PDZ domain of …
Crystal structures of the photosystem II D1 C-terminal processing protease
WebFeb 2, 1996 · Wild-type cytochrome b 562 is not cleaved by Tsp in vitro (Fig. 1 A) and is expressed at comparable levels in tsp + and tsp-cells as determined by heme absorbance … WebFeb 1, 1996 · Cytochrome b562 is not cleaved by the tail-specific protease Tsp in vitro or in the periplasm of Escherichia coli but becomes a good substrate when the C-terminal sequence WVAAA is added. Following randomization of the final three residue positions of this substrate, 54 different mutants with single residue substitutions were recovered. cherne test ball plug
Full article: Degradation of C-terminal tag sequences on domain ...
WebThe Escherichia coli protease Prc (Tsp) exhibits specificity in vitro for proteins with nonpolar carboxyl termini. To determine whether Prc is responsible for the selective degradation in … WebOct 28, 2016 · Humanized Fab′ fragments may be produced in the periplasm of Escherichia coli but can be subject to degradation by host cell proteases. In order to increase Fab′ … WebFeb 16, 1996 · keiler c. k., identification of active-site residues of the tsp protease, journal of biological chemistry 270, 28864 (1995). google scholar. 15. keiler c. k., c-terminal specific … flights from lax to mankato mn